이준혁 교수

Jun Hyuck Lee

INFO

  • 소속캠퍼스극지연구소
  • 전공 극지과학
  • 직급전공책임교수
  • 연락처032-760-5555
  • 출신전공생명과학과
  • 학위박사
  • 최종출신대학광주과학기술원
  • 이메일

연구분야

저온 활성단백질의 구조-기능 연구

Structural and functional studies on cold-active proteins

대표연구실적

-  Do H, Kim S-J, Kim HJ, Lee JH*. Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein fromthe Antarctic bacterium Flavobacterium frigoris PS1. Acta Crystallogr D Biol Crystallogr. 2014, D70, 1061-1073 -  Lee JH*, Park AK*, Do H, Park KS, Moh SH, Chi YM, Kim HJ. Structural basis for the antifreeze activity of an ice-binding protein from an Arctic yeast. J Biol Chem. 2012;287;11460-11468 -  Lee JH, Rangarajan ES, Yogesha SD, Izard T. Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions. Structure. 2009;17:833-842.

논문(최근 5년)

-  Ice-Binding Protein Derived from Glaciozyma Can Improve the Viability of Cryopreserved Mammalian Cells[JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2015-12-28] -  Structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica[SCIENTIFIC REPORTS,2016-01-18] -  Structural Insights into the Quaternary Catalytic Mechanism of Hexameric Human Quinolinate Phosphoribosyltransferase, a Key Enzyme in de novo NAD Biosynthesis[SCIENTIFIC REPORTS,2016-01-25] -  Crystal structure and modeling of the tetrahedral intermediate state of methylmalonate-semialdehyde dehydrogenase (MMSDH) from Oceanimonas doudoroffii[JOURNAL OF MICROBIOLOGY,2016-02-01] -  Molecular mechanism of constitutively active Rab11A was revealed by crystal structure of Rab11A S20V[FEBS LETTERS,2016-03-01] -  Complete genome sequence of the crude oil-degrading thermophilic bacterium Geobacillus sp. JS12[ JOURNAL OF BIOTECHNOLOGY,2016-07-20] -  Crystal Structure of Cytochrome P450 (CYP105P2) from Streptomyces peucetius and Its Conformational Changes in Response to Substrate Binding[INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016-05-25] -  Crystal structures of aldehyde deformylating oxygenase from Limnothrix sp. KNUA012 and Oscillatoria sp. KNUA011[ BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016-08-26] -  Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica[SCIENTIFIC REPORTS,2016-09-22] -  Proteomic and transcriptomic investigations on cold-responsive properties of the psychrophilic Antarctic bacterium Psychrobacter sp. PAMC 21119 at subzero temperatures[ENVIRONMENTAL MICROBIOLOGY,2017-02-01] -  Biophysical characterization of soluble Pseudomonas syringae ice nucleation protein InaZ fragments[ INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017-01-01] -  Controlled dehydration improves the diffraction quality of two RNA crystals[ BMC STRUCTURAL BIOLOGY,2016-11-03] -  Crystal Structure of a Putative Cytochrome P450 Alkane Hydroxylase (CYP153D17) from Sphingomonas sp. PAMC 26605 and Its Conformational Substrate Binding[INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016-12-09] -  Characterization of Two Antimicrobial Peptides from Antarctic Fishes (Notothenia coriiceps and Parachaenichthys charcoti)[PLOS ONE,2017-01-25] -  Crystal Structure and Functional Characterization of an Esterase (EaEST) from Exiguobacterium antarcticum[PLOS ONE,2017-01-26] -  Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant[MARINE DRUGS,2017-01-27] -  Crystal structure of the inactive state of the receiver domain of Spo0A from Paenisporosarcina sp. TG-14, a psychrophilic bacterium isolated from an Antarctic glacier[JOURNAL OF MICROBIOLOGY,2017-06-01] -  The genome of the Antarctic-endemic copepod, Tigriopus kingsejongensis[GIGASCIENCE,2017-01-01] -  Crystal Structure and Functional Characterization of a Cytochrome P450 (BaCYP106A2) from Bacillus sp. PAMC 23377[JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2017-08-28] -  Crystal structure and functional characterization of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea strain 34H[PLOS ONE,2017-07-19] -  Crystal structure of cis-dihydrodiol naphthalene dehydrogenase (NahB) from Pseudomonas sp. MC1: Insights into the early binding process of the substrate[ BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017-09-16] -  Crystal structure of 5-enolpyruvylshikimate-3-phosphate synthase from a psychrophilic bacterium, Colwellia psychrerythraea 34H[ BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017-10-21] -  Improving thermal hysteresis activity of antifreeze protein from recombinant Pichia pastoris by removal of N-glycosylation[ PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2017-03-16] -  Interaction mode of CIDE family proteins in fly: DREP1 and DREP3 acidic surfaces interact with DREP2 and DREP4 basic surfaces[PLOS ONE,2017-12-14] -  Pilot-scale Production of Recombinant LeIBP from Arctic Yeast Glaciozyma sp. by Escherichia coli[KSBB Journal,2017-11-28] -  PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis[ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2018-06-01] -  Identification, characterization, immobilization, and mutational analysis of a novel acetylesterase with industrial potential (LaAcE) from Lactobacillus acidophilus[BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2018-01-01] -  Crystal structure of unphosphorylated Spo0F from Paenisporosarcina sp. TG-14,a psychrophilic bacterium isolated from an Antarctic glacier[Biodesign,2018-12-30] -  Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy[CRYOBIOLOGY,2018-10-05] -  Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus[JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018-09-25] -  Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus[MARINE GENOMICS,2018-03-26] -  Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4A resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme[PLOS ONE,2018-02-21] -  Crystal structure and enzymatic properties of chalcone isomerase from the Antarctic vascular plant Deschampsia antarctica Desv.[PLOS ONE,2018-02-02] -  Crystal structure and functional characterization of a cold-active acetyl xylan esterase (PbAcE) from psychrophilic soil microbe Paenibacillus sp.[PLOS ONE,2018-10-31] -  Crystal structure of dihydrodipicolinate reductase (PaDHDPR) from Paenisporosarcina sp. TG-14: structural basis for NADPH preference as a cofactor[SCIENTIFIC REPORTS,2018-05-21] -  Characterization and mutation anaylsis of a cold-active bacterial hormonesensitive lipase from Salinisphaera sp. P7-4[ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2019-01-14] -  Increased Productivity and Antifreeze Activity of Ice-binding Protein from Flavobacterium frigoris PS1 Produced using Escherichia coli as Bioreactor[APPLIED BIOCHEMISTRY AND MICROBIOLOGY,2019-04-22] -  Characterization of Four Liver-Expressed Antimicrobial Peptides from Antarctic Fish and Their Antibacterial Activity[APPLIED SCIENCES-BASEL,2019-10-13] -  Modulating α-synuclein fibril formation using DNA tetrahedron nanostructures[BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2018-09-29] -  A novel enantioselective SGNH family esterase (NmSGNH1) from Neisseria meningitides: Characterization, mutational analysis, and ester synthesis[BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,2019-07-17] -  Structural and functional analysis of a dimeric fumarylacetoacetate hydrolase (EaFAH) from psychrophilic Exiguobacterium antarcticum[BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019-01-08] -  Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14[BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019-04-05] -  Molecular Characterization of a Novel Cold-Active Hormone-Sensitive Lipase (HaHSL) from Halocynthiibacter Arcticus[BIOMOLECULES,2019-11-05] -  Diketopiperazine and alloxazine alkaloids from the antarctic bacteria, Pseudorhodobacter psychrotolerans sp. nov[BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2019-04-28] -  Characterization of two steroid hydroxylases from different Streptomyces spp. and their ligand-bound and -unbound crystal structures[FEBS JOURNAL,2019-05-01] -  Characterization, immobilization, and mutagenesis of a novel cold-active acetylesterase (EaAcE) from Exiguobacterium antarcticum B7[INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019-06-21] -  Crystal Structure and Functional Characterization of a Xylose Isomerase (PbXI) from the Psychrophilic Soil Microorganism, Paenibacillus sp.[JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2019-01-01] -  Structural transformation-mediated dimerization of caspase recruitment domain revealed by the crystal structure of CARD-only protein in frog virus 3[JOURNAL OF STRUCTURAL BIOLOGY,2019-01-06] -  Structural insights into the enzyme specificity of a novel ω-transaminase from the thermophilic bacterium Sphaerobacter thermophilus[JOURNAL OF STRUCTURAL BIOLOGY,2019-09-24] -  Structural and functional characterization of a novel cold?active S?formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium[MICROBIAL CELL FACTORIES,2019-08-19] -  The complete mitogenome of the Arctic moss Aulacomnium turgidum (Wahlenb.) Schwaegr[MITOCHONDRIAL DNA PART B-RESOURCES,2019-09-23] -  Complete genome sequencing of Shigella sp. PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation[MICROBIAL PATHOGENESIS,2019-09-24] -  Antarctic blackfin icefish genome reveals adaptations to extreme environments[NATURE ECOLOGY & EVOLUTION,2019-03-01] -  Type II Ice-Binding Proteins Isolated from an Arctic Microalga Are Similar to Adhesin-Like Proteins and Increase Freezing Tolerance in Transgenic Plants[PLANT AND CELL PHYSIOLOGY,2019-08-16] -  Structural basis of small RNA hydrolysis by oligoribonuclease (CpsORN) from Colwellia psychrerythraea strain 34H[SCIENTIFIC REPORTS,2019-02-25] -  Nanopore sequencing reads improve assembly and gene annotation of the Parochlus steinenii genome[SCIENTIFIC REPORTS,2019-03-25] -  Structural basis of substrate recognition by a novel thermostable (S)-enantioselective ω-transaminase from Thermomicrobium roseum[SCIENTIFIC REPORTS,2019-05-06] -  Structural analysis of a novel substrate-free form of the aminoglycoside 600-N-acetyltransferase from Enterococcus faecium[ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2020-07-16] -  Crystal structure of the reactive intermediate/imine deaminase A homolog from the Antarctic bacterium Psychrobacter sp. PAMC 21119[BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020-02-12] -  Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH?type lipase (HaSGNH1) from the psychrophilic bacterium Halocynthiibacter arcticus[BIOTECHNOLOGY FOR BIOFUELS,2020-03-16] -  Comparative Genomic Study of Polar Lichen?Associated Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 Reveals the Presence of Polysaccharide?Degrading Ability Based on Habitat[CURRENT MICROBIOLOGY,2020-07-17] -  TWO NEW PHENOLIC COMPOUNDS FROM THE ANTARCTIC LICHEN Pertusaria dactylina[CHEMISTRY OF NATURAL COMPOUNDS,2020-02-04] -  Crystallization and Preliminary X-ray Diffraction Study of a Novel Bacterial Homologue of Mammalian Hormone-Sensitive Lipase (halip1) from Halocynthiibacter arcticus[CRYSTALS,2020-10-23] -  The Genome Assembly and Annotation of the Southern Elephant Seal Mirounga leonina[GENES,2020-02-03] -  Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach[GENES & GENOMICS,2020-07-31] -  Structural and sequence comparisons of bacterial enoyl-CoA isomerase and enoyl-CoA hydratase[JOURNAL OF MICROBIOLOGY,2020-03-30] -  Structural insights into the psychrophilic germinal protease PaGPR and its autoinhibitory loop[JOURNAL OF MICROBIOLOGY,2020-07-15] -  Enzymatic Synthesis of Anabolic Steroid Glycosides by Glucosyltransferase from Terribacillus sp. PAMC 23288[JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2019-12-30] -  Chromosomal-level assembly of Takifugu obscurus (Abe, 1949) genome using third-generation DNA sequencing and Hi-C analysis[MOLECULAR ECOLOGY RESOURCES,2020-03-01] -  A New Chlorinated Phenolic Compound From the Antarctic Lichen, Pertusaria dactylina[NATURAL PRODUCT COMMUNICATIONS,2019-12-26] -  The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification[THERIOGENOLOGY,2020-04-17] -  Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation[VETERINARY MEDICINE AND SCIENCE,2020-08-01]

특허(최근 5년)

-  루코스포리디움 속 미생물 유래 단백질을 포함하는 동결 보존용 조성물 및 이를 이용한 동결 손상 방지 방법 [2017-08-02]

연구과제 수행 실적

극지유전체 101 프로젝트: 극지생물 유전체 정보 분석 및 활용기반 구축 북극권 동토 관측 거점유래 고분자 유기 탄소 화합물 분해관련 유용 저온효소 활용연구